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Bringing Soil Animals to Life

How can we involve and educate people as to the wonders of life in the soil? While we have
filmed every other frontier, our earth has been impenetrable.

How can we bring the animals in the soil to life? Below it the proper gear for extarcting small soil creature. They are called Tullgren Funnels. 
Tullgren Funnels
 It came to me when trying to explain to some youngsters around the house. I picked up a lump of soil, and said: "Inside this there are lots of little animals which I can show you. If I can get these creatures to move out of the soil I can show you what they look like" And this is how to do it on the cheap...
There are thanks to several people in helping me to overcome some challenges - Keith for getting the creatures onto tissue, Nancy for tin can idea, and Rob for suggesting to strap digital microscope to binocular microscope. 

Recipe

Ingredients

Any lump of soil whether cold of clay, leaf litter or bit of lawn..

Empty bottle 2 litre cola

Tin can

1 Light Bulb 50-100 watt, NOT energy saving! You need an old one that throws off some heat.

Water

Binocular Microscope (£100) , or 20-200X digital microscope that show on PC screens £20+)

Retort Stand (or just put upturn the Cola bottle in a jam jar) to hold Cola bottle.

Gauze, either metal or cotton


Method
I made a 60 Tullgren funnel unit. So thought I'd buy a unit lik eone above. Expect when it cam eto the grand needed. So I thought I'd do it on the cheap...
1. Use a tin can as a little auger (soil sampler). Cut off both ends (some cans netter to remove both ends cleanly. 
One soil Auger
Push it in to the ground as you want to sample down about 2 inches. Swirl tin round with your hands to sever sample from rest of soil. Pull this out of the ground, then push the clod to the other end of the can. Now, what was the surface is showing from the bottom of the can. Put some cotton gauze over the end with the soil surface and tape it on. Then put into this...



2. Extraction Apparatus. Cut a large cola bottle horizontally in half. Tip it upside down so the cap is at the bottom and arrange a 
Cheap Extraction equipment
way to hold it steady - like the jam jars here and retort stand.  Upturn the tin can so that the soil surface is now face down. Put an old fashioned bulb about 2 inches above. The tin can is important to hold the soil, as it conducts heatr away and allows a temperature gradient from top to bottom to build up.  It is that temperature difference that drives the arthropods away from the heat, down tohe bottle and into th vials with water below.

3. Put light on for several days. I used to do this with 60 funnels for a week or two on end, and never worry about the electrics and possibility of of a fire. Now I am careful to leave one bulb on overnight.  The creatures come out at different rates..clips of 'coming thursday etc'tin funnel

4. The vials hold the creatures for a few days. Use the endoscope microscop to look into the vial to see what is going on the surafce of the water. The minscus hodls the creatures together. When you are ready, pour on to tissue in a petri duish Use a (20-200X digital microsope) - where you can view the image on screen.  
easier to stay in focus, and move the paper round. And the creatures walk around much more like they are in the soil, some burrowing through, others climbing over ridges. And they are in complete colour, so very much more like the conditions in which they live, and we can see them move as they would in the soil. 

Have a look at two sample videos, based on the extraction process described above, collected on the first day. Access them (in full colour!) in Dropbox, Soilanimals 1.1 & Soilanimals 1.2 (See file at bottom of page, 'Soilanimals 1 & 3', on how to 'read' these videos)

First are springtails..
Later the oribatid mites appear. there are different stages in their life going from nymphs to hard nut adults, which eat into leaves. The Soil Report 2007 by CS noted that "highest catches of mites were found in plots at which rainforest was highest. while springtails favoured lower rainfall plots" and "Highest mite:springtail were found in habitats that are associated with high rain fall". (8.3.4)
Present
This is what home soil lab look slike..
Home soil science lab

Then going to Todmorden

Nematodes
Nematodes require different ways to extract,
Nematode extraction Let sit 1-3 days to allow the nematodes to crawl out of the soil. Make sure the sample stays in contact with the water - do not let the dish become dry. Covering the dish with plastic wrap or foil will help prevent drying. This tells us something about nematodes - that they cannot exist outside water. Except when they make cysts, which withstand all sorts of conditions.

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